Comprehensive Transcriptomic Analysis of BV2 Microglia Following TDP-43 Knockdown Versus Non-Silencing Control

ABSTRACT: In this study, we investigate how knockdown of the RNA-binding protein TDP-43 alters the transcriptional program of BV2 microglial cells. Aberrant TDP-43 function is a hallmark of neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD), and while most research has focused on neurons, emerging evidence points to a critical role for microglia in modulating neuroinflammation and disease progression. We employed RNA interference to specifically deplete TDP-43 in BV2 cells (TDP-43 RNAi) and compared them to cells treated with a non-silencing control siRNA. After 24 hours of knockdown, total RNA was isolated. Libraries were prepared from 500 ng of total RNA using the SEQuoia Complete Stranded RNA Library Prep Kit with ribosomal depletion using the

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Mus musculus

SUBMITTER: Ismail Gbadamosi 

PROVIDER: E-MTAB-15184 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress