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HP1, H3-K27me3, and H3-K9me3 Cut & Tag of Het (Cbx1+/+, Cbx3+/+, Cbx5+/-) and TKO (Cbx1-/-, Cbx3-/-, Cbx5-/-) bipotential mouse embryonic liver (BMEL) cells

ABSTRACT: Cut & Tag was used to localize the 3 mouse HP1 proteins (Cbx1,Cbx3,Cbx5) to the genome of BMEL (bipotential mouse embryonic liver) cells. Het BMEL cells are Cbx1+/+, Cbx3+/+, Cbx5+/- HP1-TKO (triple knock-out cells) are Cbx1-/-, Cbx3-/-, Cbx5-/-. The HP1_TKO cells were used to verify the specificity of the Cut & Tag signals. HP1 localization was compared with the chromatin localization of select RNA exosome subunits and adapters to support a role for HP1 in targeting the RNA exosome to specific chromatin regions.

INSTRUMENT(S): NextSeq 550

ORGANISM(S): Mus musculus

SUBMITTER: Carl MANN

PROVIDER: E-MTAB-15189 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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