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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling by array of mouse amygdala to detect transcripts regulated by psychological stress

ABSTRACT: Detection of transcripts regulated by psychological stress in mouse amygdala. Prior to experiments mice were kept undisturbed for one week in their home cages. Restraint stress was performed during the light period of the circadian cycle. Control animals were left undisturbed and stressed animals were subjected to a six hour restraint stress session in a separate room. The mice were placed in their home cages in wire mesh restrainers secured at the head and tail ends with clips

ORGANISM(S): Mus musculus

SUBMITTER: Nathan Skene

PROVIDER: E-MTAB-1519 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Stress-induced lipocalin-2 controls dendritic spine formation and neuronal activity in the amygdala.

Skrzypiec Anna E AE Shah Rahul S RS Schiavon Emanuele E Baker Eva E Skene Nathan N Pawlak Robert R Mucha Mariusz M

PloS one 20130409 4

Behavioural adaptation to psychological stress is dependent on neuronal plasticity and dysfunction at this cellular level may underlie the pathogenesis of affective disorders such as depression and post-traumatic stress disorder. Taking advantage of genome-wide microarray assay, we performed detailed studies of stress-affected transcripts in the amygdala - an area which forms part of the innate fear circuit in mammals. Having previously demonstrated the role of lipocalin-2 (Lcn-2) in promoting s ...[more]

PMID: 23593384

Similar Datasets

Transcription profiling by array of mouse amygdala to detect transcripts regulated by psychological stress

Project description:Detection of transcripts regulated by psychological stress in mouse amygdala. Prior to experiments mice were kept undisturbed for one week in their home cages. Restraint stress was performed during the light period of the circadian cycle. Control animals were left undisturbed and stressed animals were subjected to a six hour restraint stress session in a separate room. The mice were placed in their home cages in wire mesh restrainers secured at the head and tail ends with clips

2015-10-23 | E-MTAB-1519 | ExpressionAtlas

Biomarkers of hippocampal gene expression in a mouse restraint chronic stress model

Project description:Acute stress provides many beneficial effects whereas chronic stress contributes to a variety of human health problems. The objective of this study was to use a rodent model to uncover hippocampal gene signatures associated with prolonged chronic stress which could potentially serve as biomarkers and therapeutic targets for early diagnosis and pharmacological intervention for stress induced disease. Mice were subjected to restraint stress over 7 consecutive days and gene expression changes in the hippocampus were analyzed at 3, 12 and 24 hours following the final restraint treatment. Data indicated that mice exposed to chronic restraint stress exhibit a differential gene expression profile compared to non-stressed controls. The greatest differences were observed 12 and 24 hrs following the final stress test.

2016-11-02 | E-MTAB-2402 | biostudies-arrayexpress

Transcription profiling of mouse liver after combined acoustic and restraint stress compared to non-stressed control mice

Project description:Stress is a powerful modulator of neuroendocrine, behavioral and immunological functions. After 4.5 days of repeated combined acoustic and restraint stress as a murine model of chronic psychological stress severe metabolic dysregulations became detectable in female BALB/c mice. Stress-induced alterations of metabolic processes that were found in a hepatic mRNA expression profiling were verified by in vivo analyses. Repeatedly stressed mice developed a hypermetabolic syndrome with severe loss of lean body mass, hyperglycemia, dyslipidemia, increased amino acid turn-over, and acidosis. This was associated with hypercortisolism, hyperleptinemia, insulin resistance, and hypothyroidism. In contrast, after a single acute stress exposure changes in expression of metabolic genes were much less pronounced and predominantly confined to gluconeogenesis, probably indicating that metabolic disturbances might be initiated already early but will only manifest in repeatedly stressed mice .Thus, in our murine model, repeated stress caused severe metabolic dysregulations leading to a drastic reduction of the individualâs energy reserves. Under such circumstances stress may further reduce the ability to cope with new stressors such as infection or cancer. Endocrinology Epub ahead of print, March 11, 2008; doi:10.1210/en.2008-0038; This SuperSeries is composed of the following subset Series:; GSE11123: Mouse liver gene expression after a single acute 2h exposure to combined acoustic and restraint stress vs. control; GSE11125: Mouse liver gene expression after 4.5 days of repeated combined acoustic and restraint stress vs. control Experiment Overall Design: Refer to individual Series

2008-06-18 | E-GEOD-11126 | biostudies-arrayexpress

Transcription profiling of mouse liver after 4.5 days of repeated combined acoustic and restraint stress vs. control

Project description:Stress is a powerful modulator of neuroendocrine, behavioral and immunological functions. After 4.5 days of repeated combined acoustic and restraint stress as a murine model of chronic psychological stress severe metabolic dysregulations became detectable in female BALB/c mice. Stress-induced alterations of metabolic processes that were found in a hepatic mRNA expression profiling were verified by in vivo analyses. Repeatedly stressed mice developed a hypermetabolic syndrome with severe loss of lean body mass, hyperglycemia, dyslipidemia, increased amino acid turn-over, and acidosis. This was associated with hypercortisolism, hyperleptinemia, insulin resistance, and hypothyroidism. In contrast, after a single acute stress exposure changes in expression of metabolic genes were much less pronounced and predominantly confined to gluconeogenesis, probably indicating that metabolic disturbances might be initiated already early but will only manifest in repeatedly stressed mice .Thus, in our murine model, repeated stress caused severe metabolic dysregulations leading to a drastic reduction of the individualâs energy reserves. Under such circumstances stress may further reduce the ability to cope with new stressors such as infection or cancer. Endocrinology Epub ahead of print, March 11, 2008; doi:10.1210/en.2008-0038 Experiment Overall Design: Two biological experiments of repeated combined acoustic and restraint stress were performed, which consist each of a repeatedly stressed group and an untreated control group. Liver RNA expression profiles were analyzed in technical duplicates using pools of 8 or 9 individual RNAs of each group.

2008-06-18 | E-GEOD-11125 | biostudies-arrayexpress

Transcriptomics study of the effect of stress and Nitric oxide synthase inhibition in a mouse model of breast cancer

Project description:We describe the effects of restraint stress in presence or absence of an inhibitor of nitric oxide synthase on metastasis formation in lungs of in vivo syngeneic model of mouse breast cancer analysed by whole genome expression microarrays

2019-09-26 | E-MTAB-7299 | biostudies-arrayexpress

Transcription profiling of mouse liver gene expression after a single acute 2h exposure to combined acoustic and restraint stress vs. control

Project description:Stress is a powerful modulator of neuroendocrine, behavioral and immunological functions. After 4.5 days of repeated combined acoustic and restraint stress as a murine model of chronic psychological stress severe metabolic dysregulations became detectable in female BALB/c mice. Stress-induced alterations of metabolic processes that were found in a hepatic mRNA expression profiling were verified by in vivo analyses. Repeatedly stressed mice developed a hypermetabolic syndrome with severe loss of lean body mass, hyperglycemia, dyslipidemia, increased amino acid turn-over, and acidosis. This was associated with hypercortisolism, hyperleptinemia, insulin resistance, and hypothyroidism. In contrast, after a single acute stress exposure changes in expression of metabolic genes were much less pronounced and predominantly confined to gluconeogenesis, probably indicating that metabolic disturbances might be initiated already early but will only manifest in repeatedly stressed mice .Thus, in our murine model, repeated stress caused severe metabolic dysregulations leading to a drastic reduction of the individualâs energy reserves. Under such circumstances stress may further reduce the ability to cope with new stressors such as infection or cancer. Endocrinology Epub ahead of print, March 11, 2008; doi:10.1210/en.2008-0038 Experiment Overall Design: Two biological experiments of a single acute exposure to combined acoustic and restraint stress were performed, which consist each of an acutely stressed group and an untreated control group. Liver RNA expression profiles were analyzed using pools of 8 or 9 individual RNAs of each group.

2008-06-18 | E-GEOD-11123 | biostudies-arrayexpress

Expression data from sleep deprivation experiment in mouse hippocampus

Project description:We used microarrays to detail the global programme of gene expression underlying the effect of sleep deprivation in the mouse hippocampus and identified distinct classes of regulated genes during this process. Hippocampal tissue was taken from sleep deprived mice and time-matched non-sleep-deprived control animals that were left undisturbed in their home cages during the sleep deprivation period for a total of 8 and 9 replicates per group. RNA was isolated and cDNA was synthesized from hippocampal tissue, and the sample from each animal was hybridized to a separate Affymetrix Mouse 430_2 microarray

2012-09-06 | E-GEOD-33302 | biostudies-arrayexpress

Alterations in the gene expression pattern of the pituitary is consistent with the domesticated phenotype in chickens

Project description:Domesticated animals share a unique set of morphological and behavioural traits, jointly referred to as the domesticated phenotype. These include modified growth, reproduction, metabolism, pubertal development and stress response. Striking similarities amongst a range of unrelated domesticated species suggest that similar regulatory mechanisms may underlie the domesticated phenotype. Several previous studies have focused on the brain to find mechanisms underlying domestication effects on the stress response, whereas the potential role of the pituitary gland as a target of domestication is highly overlooked. Here, we study gene expression in the pituitary gland of the domesticated White Leghorn chicken and its ancestor, the Red Junglefowl. We exposed chicken of both breeds to 15 minutes of restraint stress. We then culled the animals and dissected out the pituitary gland and snap froze them. RNA was later extracted from the pituitaries and gene expression was measured using Agilent microarray.

2018-03-31 | E-MTAB-6269 | biostudies-arrayexpress

Epigenetic Transgenerational Alterations to Stress Response in Brain Gene Networks and Behaviour

Project description:Ancestral environmental exposures that promote epigenetic transgenerational inheritance influence all aspects of an individual’s life history. Stress experienced during adolescence can affect adult physiological and behavioural phenotypes. The current study utilized a systems biology approach to investigate the interactions of these two forms of epigenetic modification, one carried in the germline transgenerationally and the other contained in the context of life history. A transgenerational epigenetic imprint left by the fungicide vinclozolin promoted regional specific brain gene networks that influenced chronic restraint stress responses to alter adult physiological, brain and behavioural phenotypes. The environmentally-induced epigenetic transgenerational inheritance was found to interact with early life stress response to impact the adult brain genome activity to bring “the phenotype into being”. We used microarrays to determine genes expressed differentially in rats' 4 brain areas - basolateral amygdala (BLA), primary and secondary motor cortex (Crtx), CA1 of the hippocampus (CA1), and CA3 of the hippocampus (CA3) - due to Vinclozolin treatments of their grand-grandmothers and/or to stress in adolescence age. For each of 4 brain areas (BLA, CRTX, CA1, and CA3), RNA samples from 4 treatment groups - stressed control (St-Con), non-stressed control (NSt-Con), stressed vinclozolin (St-Vin), and non-stressed vinclozolin (NSt-Vin) - were compared to each other. Each treatment groups contained 3 biological replica. RNA for each replica was pooled from 4 individual animals forming a certain dyad group.

2012-02-01 | E-GEOD-26737 | biostudies-arrayexpress

Transgenerational early stress affymetrix gene expression parental chicken

Project description:Transgenerational effects of early experience on behavioural, hormonal and gene expression responses to acute stress in the precocial chicken. The data for the offspring of the birds in this experiment is available in ArrayExpress with accession E-MTAB-925.

2012-01-02 | E-MTAB-924 | biostudies-arrayexpress

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