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RNA-seq of E17.5 WT and Emb-/- kidney

ABSTRACT: To study function of Embigin in mouse embryos, five E17.5 WT and Emb-/- kidneys were dissected, and the RNA was isolated and submitted to high-throughput sequencing to find differentially expressed genes between WT and Emb knock-out mouse lungs or placentas

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Mus musculus

SUBMITTER: Pekka Rappu

PROVIDER: E-MTAB-15210 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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RNA-seq of E17.5 WT and Emb-/- mouse placentas and lungs

Project description:To study function of Embigin in mouse embryos, five E17.5 WT and Emb-/- placentas and lungs were dissected, and the RNA was isolated and submitted to high-throughput sequencing to find differentially expressed genes between WT and Emb knock-out mouse lungs or placentas

2023-08-31 | E-MTAB-10641 | biostudies-arrayexpress

RNA-seq of E13.5 WT and Emb-/- kidney

Project description:To study function of Embigin in mouse embryos, E13.5 WT (four individuals per replicate) and Emb-/- (six individuals per replicate) kidneys were dissected, and the RNA was isolated and submitted to high-throughput sequencing to find differentially expressed genes between WT and Emb knock-out mouse lungs or placentas

2026-02-10 | E-MTAB-15211 | biostudies-arrayexpress

RNA-seq of mouse keratinocytes treated with siRNA against embigin

Project description:To study the potential role of embigin in the regulation of cellular processes, we used specific siRNAs to knockdown embigin expression in mouse keratinocytes. Two independent siRNAs, Mm_Emb1 and Mm_Emb4 siRNAs, were selected for experiments.After embigin silencing, the cells were harvested and their gene expression patterns were analyzed by RNA sequencing.

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RNA-seq of E17.5 WT and Emb-/- kidney

Project description:RNA-seq of E17.5 WT and Emb-/- kidney

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Transcriptional profiling of EMB+ cells and EMB- cells across long bone and vertebrae

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Transcriptomic responses to emamectin benzoate in Pacific and Atlantic Canada salmon lice Lepeophtheirus salmonis with differing levels of drug resistance

Project description:This study investigates the baseline or inducible differences in between populations of Atlantic salmon lice Lepeophtheirus salmonis with differing levels of resistance to the parasiticidal drug emamectin benzoate (EMB), as well as the induced effects of EMB exposure to Pacific salmon lice. F1 generation lice were exposed in bioassays to a dilution series of emamectin benzoate. Two separate experiments were conducted, one for Atlantic and one for Pacific salmon lice (to be analyzed separately). Atlantic pre-adult salmon lice, separated into male and female, and sensitive or resistant to EMB populations, and exposed to a dilution series: 0 (control), 0.1, 25, 300, and 1000 parts per billion EMB. For each combination four biological replicates were included, except male resistant 25 (n = 3) and female resistant 300 (n = 2). Pacific pre-adult lice of both sexes were exposed to a dilution series: 0 (control), 25, 50 parts per billion EMB.

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TP12 adult with emb 3

Project description:Timepoint 12 wild type adult with emb 3 vs mixed stage reference Keywords: other

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TP11 adult with emb 2

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Effect of genetic knockdown Emb on enteric neuronal development in mice

Project description:EMB is a neural adhesion molecule that participates in the development of the enteric nervous system, but its specific mechanism in the development of enteric neurons remains unclear. We constructed Emb knockdown mice and performed SmartseqII transcriptome sequencing by flow sorting CD56+CD24+ intestinal neurons from 4-week-old wild-type and Emb knockdown mice. This work helps us further understand the role of EMB proteins in the development of intestinal neurons.

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Myocardial Gene Expression Profiling to Predict and Identify Cardiac Allograft Acute Cellular Rejection

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