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RNA-seq of mouse fetal intestines comparing wildtype littermates with Notch inhibited mutants

ABSTRACT: Whole intestines were harvested from fetuses from timed pregnant matings for wildtype littermates and endothelial specific Notch inhibited mutants (Jax strain #032613). Fetal stages were confirmed according to the Theiler staging chart (https://www.emouseatlas.org/emap/ema/staging_criteria/staging_criteria.html). Whole intestines (from the common bile duct through the cecum) were collected at E16-E16.5. Male and female intestines from each stage were pooled and dissociated to single cells for single cell RNA sequencing as previously described (Miller et al. 2020 Dev Cell). 705-KW-1 and 705-KW-2 were whole intestine. 2482-KW1 and 2482-KW2 samples were enriched with CD31+ MACS beads. 3593-KW1 and 3593-KW2 samples were enriched with CD31+ MACS beads and depleted for CD45.

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Mus musculus

SUBMITTER: Katherine Walton

PROVIDER: E-MTAB-15222 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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