Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Hydrogen sulfide regulation of the transcriptional profile of rice plants under drought stress

ABSTRACT: To investigate the effect of H2S on the rice transcriptomic responses to drought stress, we performed transcriptome sequencing analysis in four experimental treatments: rice watered plants (control sample, C), rice plants watered with NaHS solution, as sulfide donor (sulfide treatment, S), rice plants subjected to drought stress (drought, D) and rice plants pretreated with NaHS and subjected to drought stress (S_D). Thus, 25-day-old plants grown in soil under physiological conditions were divided into two batches with one batch irrigated with water and the other with NaHS for 10 days, replacing the NaHS solution every two days. After this period, each batch was subsequently divided into two new batches and subjected to water irrigation or drought for another 15 additional days. At the end of the full treatment, four replicates of each condition were obtained that were processed independently

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Oryza sativa Japonica Group

SUBMITTER: LUIS ROMERO

PROVIDER: E-MTAB-15224 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Project description:The urgent need to address water scarcity underscores the importance of enhancing plant drought resistance. This study investigates whether pretreatment with abscisic acid (ABA) activates early stress signaling, thereby improving barley drought response when subsequently exposed to drought conditions. Although the individual responses to drought and ABA are well-documented, their synergistic effects in barley warrant further investigation. This study examines the impact of ABA on barley drought resilience through an experimental design that incorporates four distinct treatments: optimal watering, ABA application at 60 days post-sowing, and two drought stress treatments - one with and the other without prior ABA application. Key physiological parameters, such as photosynthesis, stomatal conductance and chlorophyll content, were analyzed in conjunction with transcriptomics. The results suggest that ABA pretreatment initiates early stomatal closure and elevates the expression of essential genes like NCED1, BG8, and HvA22, priming barley for improved drought resistance. During the drought, ABA-pre-treated barley maintained high chlorophyll levels, indicating sustained photosynthetic activity, a trend that persisted across treatments during the post-drought recovery phase. Furthermore, ABA pre-treatment was found to preserve photosystem II efficiency during drought conditions. Transcriptomic analyses revealed distinct gene expression profiles, alternative splicing profile and isoform switching, highlighting the molecular complexities of ABA role in drought response. These alterations span stress response, metabolic pathways, and DNA modification processes, providing a comprehensive view of ABA treatment's regulatory and metabolic impacts. In conclusion, ABA pretreatment strengthens barley drought defense by fostering stomatal closure and gene activation, guiding research strategies grounded in ABA and suggesting that genotypes with elevated ABA levels could have enhanced resilience and recovery capabilities.

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Hydrogen sulfide regulation of the transcriptional profile of rice plants under drought stress

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