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RNA-seq of K1 papillary thyroid cancer (PTC) cell with different TSC22D1 overexpression status

ABSTRACT: RNA sequencing was performed on K1 papillary thyroid cancer (PTC) cells to investigate the biological function of TSC22D1 variant 2 overexpression. Cells were transfected with the TSC22D1 (NM_006022) Human Tagged ORF Clone, with three empty vector-transfected cells ((TSC22D1_0) serving as negative controls. Experimental groups included four replicates each of cells with low (TSC22D1_200) and high levels (TSC22D1_500) of TSC22D1 variant 2 overexpression. RNA integrity was confirmed for all samples using the Agilent TapeStation system, with RNA integrity numbers (RIN) exceeding 7.0.

INSTRUMENT(S): Not applicable in this study, Liquid nitrogen freezer, surgical instruments, Illumina NovaSeq 6000, Benchtop centrifuge, vortex, NanoDrop or plate reader (for RiboGreen assay), TapeStation (Agilent), Thermal cycler (Bio-Rad C1000), Agilent 2100 Bioanalyzer, Qubit 4 Fluorometer, Linux server, HPC cluster

ORGANISM(S): Homo sapiens

SUBMITTER: Dong Hyun Seo

PROVIDER: E-MTAB-15253 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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