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Transcriptome profiling of mammary gland cell subpopulations isolated from the transgenic mouse model FVB 11.5-kb-GFP.

ABSTRACT: The transgenic FVB/N 11.5kb-GFP mouse line was generated using the 11.5 kb s-SHIP promoter. During puberty, GFP expression in the mammary gland is specifically restricted to cap cells, which exhibit stem cell-like properties. To investigate the molecular basis underlying the differences between these basal GFP⁺ cap cells and other mammary epithelial subpopulations, we employed fluorescence-activated cell sorting to isolate four distinct mammary cell populations from 6-week-old mice, based on GFP expression in combination with CD24 and CD49f cell surface markers. These populations were subsequently analyzed for gene expression profiles.

ORGANISM(S): Mus musculus

SUBMITTER: Frederic Lepretre

PROVIDER: E-MTAB-15385 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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