SMART-seq2 of Kdm5b Overexpression in Fully Grown Oocytes

ABSTRACT: RNA-seq of single Fully Grown Oocytes using SMART-seq2. The oocytes were collected from female FVB mice, approximately. 44-48 h post-PMSG injection. The oocytes were microinjected with 1.3-1.7 μg/μL Kdm5b wild-type (WT) or catalytic mutant (CM) mRNA. Noninjected oocytes are denoted as “Noninj”. The oocytes were cultured overnight for approximately 16 h in M2 containing 20 μM Milrinone and 5% FBS and collected for sequencing.

INSTRUMENT(S): NextSeq 2000

ORGANISM(S): Mus musculus

SUBMITTER: Jasmina Al-Mousawi 

PROVIDER: E-MTAB-15453 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress