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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Time course of Embryonic Genome Activation Following Kdm5b Overexpression using SMART-seq2

ABSTRACT: RNA-seq of single mouse embryos using SMART-seq2. The embryos have been generated with IVF and are staged as hours post-IVF. At the zygote stage (~ 4h post-IVF), the embryos have been microinjected with 1.7 μg/μL Kdm5b wild-type (WT) or catalytic mutant (CM) mRNA. Non-injected embryos are denoted as “Noninj”. The embryos were cultured until collection at 20 h, 23 h, and 26 h post-IVF. A note on replicates: The replicate annotation refers to the number of times the specific condition was collected, i.e., the N number. It does not reflect the experimental batch. For the IVF batch, please refer to the sample sheet provided in the accompanying Git repository.

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Mus musculus

SUBMITTER: Jasmina Al-Mousawi

PROVIDER: E-MTAB-15454 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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