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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Single cell RNA-seq to investigate transcriptional profiles of thymic double negative T cells in WT, NPM-ALK(Tg/+) and NPM-ALK(Tg/+)Cdk6-/-Stat5b-/- mice

ABSTRACT: To examine transcriptional differences in thymic double negative (DN) T cells between wildtype (WT), NPM-ALK(Tg/+) and NPM-ALK(Tg/+)Cdk6-/-Stat5b-/- mice, single-cell RNA sequencing (scRNA-seq) was performed on fluorescence-activated cell sorted (FACS) DN T cells. The aim was to understand how genetic alterations in the NPM-ALK(Tg/+) and NPM-ALK(Tg/+)Cdk6-/-Stat5b-/- backgrounds influence early T cell development and oncogenic transformation at the transcriptional level.

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Mus musculus

SUBMITTER: Thorsten Klampfl

PROVIDER: E-MTAB-15478 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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