Unknown,Transcriptomics,Genomics,Proteomics

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Single-Cell Transcriptomics of Acetaminophen-Induced Responses in Human 2D and 3D Liver Microtissues

ABSTRACT: This study aimed to investigate how liver cell culture architecture influences acetaminophen (APAP)-induced transcriptional responses at single-cell resolution. We used human liver microtissues composed of primary human hepatocytes (PHHs), Kupffer cells (KCs), and hepatic sinusoidal endothelial cells (HSECs), cultured as 2D monolayers or 3D spheroids. Microtissues were exposed to vehicle, low-dose (350 µM), or high-dose (2687 µM) APAP for 24 hours. Single-cell suspensions were generated, and RNA libraries were prepared using the 10x Genomics Chromium platform, followed by Illumina sequencing. The resulting data were analyzed using Cell Ranger and Seurat to identify differentially expressed genes, assess pathway enrichment, and characterize cell-type–specific transcriptional responses to APAP exposure.

INSTRUMENT(S): N/A, Chromium™ Next GEM Single Cell 3′ Kit v3.1 (10X Genomics, PN-1000269), Illumina NovaSeq 6000

ORGANISM(S): Homo sapiens

SUBMITTER: Brian Bwanya

PROVIDER: E-MTAB-15516 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Single-Cell Transcriptomics of Acetaminophen-Induced Responses in Human 2D and 3D Liver Microtissues

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