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RNA sequencing of Alexander, HepG2, and Huh7 cells cultured on ESS dishes with different stiffnesses (1.5, 15, 28 kPa and reference GPa) for 48 h

ABSTRACT: To better understand how mechanical signals influence liver cancer cell behavior, we employed transcriptomic analysis using bulk RNA sequencing. Alexander, HepG2, and Huh7 cells were cultured for 48 hours on ESS dishes with varying stiffness to assess their global transcriptional responses to different extracellular matrix (ECM) conditions. Differential expression analysis revealed extensive transcriptional changes in all three cell lines, with Huh7 cells exhibiting the strongest response - demonstrated by the highest number of significantly altered genes and pathways.

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Homo sapiens

SUBMITTER: Jan Kubovčiak

PROVIDER: E-MTAB-15569 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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