Culture condition comparisons of WTC11 hiPSC by single-cell RNAseq and featuring differences between cE8, hE8 and B8+ media
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ABSTRACT: This single-cell RNA-sequencing experiment was performed for the study “Refined and benchmarked homemade media for cost-effective, weekend-free human pluripotent stem cell culture.” Human induced pluripotent stem cells (hiPSCs) from the WTC-11 line were adapted to three defined media: cE8, hE8, and B8+. Two adaptation rounds for each medium were analyzed. The goal of this experiment is to assess the heterogeneity of hiPSCs cultured in three media and whether the weekend-free culture schedule affects this heterogeneity.
INSTRUMENT(S): NextSeq 2000
ORGANISM(S): Homo sapiens
SUBMITTER: Elisa Balmas
PROVIDER: E-MTAB-15570 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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