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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Methylation arrays (Infinium Mouse Methylation Beadchip) of microglia from young and old mice subject to microglial replacement via PLX diet

ABSTRACT: Microglial replacement is emerging as a promising approach for treating age-related disorders, but effects on epigenetic age remain unclear. We examined DNA methylation changes in mouse microglia influenced by age and microglial replacement using Infinium Mouse Methylation BeadChips. We analyzed the FACS-sorted microglia of young (N=4), old (N=4) and old with microglial replacement (N=4) achieved using a PLX diet. Old microglia showed an aged epigenetic profile. Microglial depletion/repopulation, driving proliferation, led to accelerated epigenetic age. However, at the genome-wide level, repopulation reversed many aging-related changes, particularly those linked to immune and inflammatory response.

ORGANISM(S): Mus musculus

SUBMITTER: Mario Fraga

PROVIDER: E-MTAB-15631 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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