Autosomal Dosage Compensation in Aneuploidy and Cancer Is Mediated by Transcriptional Bursting Regulation (bulk RNA-Seq)

ABSTRACT: Aneuploidy, resulting from partial (segmental) or complete chromosomal gains or losses, is generally detrimental to organismal fitness, but is also associated with tumorigenicity. While sex chromosome aneuploidies are well-tolerated via dedicated allelic dosage compensation mechanisms, the existence of similar regulatory processes for autosomes remains debated. Here, we investigated transcriptional responses to autosomal aneuploidies, demonstrating the existence of autosomal dosage compensation. Using high-sensitivity, allele-resolved single-cell RNA-seq on monoclonal cell expansions with varying ploidies and degrees of autosomal aneuploidy, we uncover consistent transcriptional compensation upon chromosome losses via increased burst frequency from the remaining allele. This operates in a

INSTRUMENT(S): NextSeq 550

ORGANISM(S): Mus musculus

SUBMITTER: Sebastian Wettersten 

PROVIDER: E-MTAB-15633 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress