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GLORI mapping of m⁶A sites in human primary CD8⁺ T cells under activation and METTL3 inhibition

ABSTRACT: Glyoxal and nitrite-mediated deamination of unmethylated adenosines (GLORI) was applied to human primary CD8⁺ T cells subjected to in vitro activation with αCD3/αCD28 beads. To identify N⁶-methyladenosine (m⁶A) sites at single-nucleotide resolution and to test their dependence on METTL3, we generated GLORI libraries from non-activated (NoAct) CD8⁺ T cells, Day1-activated T cells treated for 24 hours with a competitive METTL3 inhibitor (4 μM), and Day5-activated T cells treated with the same inhibitor for 5 days. These datasets provide high-resolution m⁶A maps and allow comparison of m⁶A regulation in the presence or absence of METTL3 activity during distinct states of CD8⁺ T cell activation.

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Homo sapiens

SUBMITTER: Jernej Ule

PROVIDER: E-MTAB-15649 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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