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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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RNA-seq experiment of V79 cells treated with UV light and incubated either in monoculture or coculture with untreated cells

ABSTRACT: UV-treated cells were stained in green and seeded in density-matched monoculture with equally treated cells or in co-culture with untreated cells. The cultures were kept for 12 h prior to cell sorting and RNA isolation for bulk RNA sequencing. We wanted to compare transcriptional signatures of the UV-treated cells incubated in co-culture with the control set kept in monocultre, because the two cellular populations dispalyed morphologically different response to UV.

INSTRUMENT(S): Qiagen RNEasy Mini ki, NA, Illumina NovaSeq 6000, poly-T oligo-attached magnetic beads, BD FACSAriaIIu cell sorter

ORGANISM(S): Cricetulus griseus

SUBMITTER: Jelena Budimir

PROVIDER: E-MTAB-15733 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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