RNA-seq of E. coli MG1655 under temperature shocks, antibiotics, and combined treatments
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ABSTRACT: Bacteria experience concurrent stresses in nature; here we tested how temperature shifts modulate antibiotic responses in Escherichia coli. We profiled RNA-seq of Escherichia coli K-12 MG1655 grown in LB (37 °C, 250 rpm) and exposed to cold shock (15 °C), heat shock (42 °C), antibiotics (norfloxacin 0.10 μg/mL, ofloxacin 0.5 μg/mL, rifampicin 2.5 μg/mL), and combinations (temperature + antibiotic). Cultures were sampled at mid-exponential phase; growth was monitored and RNA was harvested 160 min after stress. The Conditions include: Standard, cold shock (CS), heat shock (HS), NOR, OFL, RIF, NOR+CS, OFL+CS, RIF+CS, NOR+HS, OFL+HS, RIF+HS (3 biological replicates each). Libraries were prepared from total RNA using bacterial rRNA depletion and sequenced as paired-end reads.
INSTRUMENT(S): Illumina NovaSeq 6000
ORGANISM(S): Escherichia coli str. K-12 substr. MG1655
SUBMITTER: Rahul Jagadeesan
PROVIDER: E-MTAB-15747 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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