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Binding sites of Fritillaria borealis U2AF2 expressed in HEK293T cells

ABSTRACT: The goal of the experiment is to reveal RNA binding preferences of Fritillaria borealis U2AF2 paralogues, using CLIP-seq. Coding sequences of U2AF2alpha, U2AF2beta and Env negative control were fused to a C-terminal V5 tag. Protein expression constructs were transfected in mammalian cell culture. After cross-linking, RNA-protein complexes were recovered with anti-V5 magnetic beads.

INSTRUMENT(S): Bio-Rad Mini-PROTEAN electrophoresis system and wet transfer cassettes,, NextSeq 500, Bio-Link Crosslinker BLX-E254

ORGANISM(S): Homo sapiens

SUBMITTER: Simon Henriet

PROVIDER: E-MTAB-15845 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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