BRBseq of human skin-derived precursor hepatic progenitor-like cells with varying polygenic risk score for hepatic fat content exposed in vitro to vehicle-matched control medium or triggers related to MASH

ABSTRACT: 92 human skin-derived precursor (hSKP) cell lines, isolated from unique donors, were genotyped for the following SNPs that have been linked to metabolic dysfunction-associated steatotic liver disease (MASLD) onset and progression: PNPLA3 rs738409 C>G, TM6SF2 rs58542926 C>T, GCKR rs1260326 C>T and MBOAT7 rs641738 C>T. Based on carriage of these SNPs, the polygenic risk score for hepatic fat content (PRS-HFC) was calculated for each cell line and used for classification in either a low, intermediate or high risk category. From the low and high risk category, five cell lines were selected and differentiated to hepatic progenitor-like cells (hSKP-HPCs) before exposing the cells for 24 hours to either vehicle-matched control medium or a mixture of MASH-related triggers including sodium oleate,

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Homo sapiens

SUBMITTER: Alexandra Gatzios 

PROVIDER: E-MTAB-15891 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress