Role of sulfide under non photorespiratory conditions in Arabidopsis plants
Ontology highlight
ABSTRACT:
INSTRUMENT(S): Illumina NovaSeq 6000
ORGANISM(S): Arabidopsis thaliana
SUBMITTER: Angeles Aroca Aguilar
PROVIDER: E-MTAB-15916 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Similar Datasets
Project description:Hydrogen sulfide is an important signaling molecule in plants that regulates essential biological processes through protein persulfidation. Although numerous persulfidated proteins were found to be involved in photorespiration, little was known about sulfide-mediated regulation of photorespiration. Label-free quantitative proteomic analysis has revealed a high impact on the protein persulfidation level when plants grown under non-photorespiratory conditions are transferred to air, with the 97 % of total identified proteins more persulfidated under suppressed photorespiration. A detailed study of the effect of sulfide on important aspects associated with photorespiratory growth conditions has provided insight into the role of sulfide in protecting plants grown under suppressed photorespiration. In these conditions, sulfide amends the metabolic imbalance of carbon/nitrogen and restores ATP levels to concentrations similar to those in air grown conditions. Sulfide also plays an essential role in balancing the significant high level of ROS measured in plants under non-photorespiratory conditions to reach a similar cellular redox state in air-grown plants, through the regulation of antioxidative defenses. Furthermore, sulfide regulates another important characteristic resulting from suppression of photorespiration, such as the stomata closure, to decrease the high guard cell ROS levels and inducing the stomata aperture. In this way, sulfide signals the movement of the CO2-dependent stomata to achieve a scenario similar to that of plants growing under normal air conditions, in the opposite direction of the already established ABA-dependent movement of the stomata. Therefore, our findings suggest that the high persulfidation level under suppressed photorespiration reveals an essential role of sulfide signaling under these conditions, and with respect to stomatal movement, the outcome of this signaling is dependent on the growth/stress condition under study.
2023-02-26 | PXD035795 | Pride
Project description:To investigate the effect of H2S on the rice transcriptomic responses to drought stress, we performed transcriptome sequencing analysis in four experimental treatments: rice watered plants (control sample, C), rice plants watered with NaHS solution, as sulfide donor (sulfide treatment, S), rice plants subjected to drought stress (drought, D) and rice plants pretreated with NaHS and subjected to drought stress (S_D). Thus, 25-day-old plants grown in soil under physiological conditions were divided into two batches with one batch irrigated with water and the other with NaHS for 10 days, replacing the NaHS solution every two days. After this period, each batch was subsequently divided into two new batches and subjected to water irrigation or drought for another 15 additional days. At the end of the full treatment, four replicates of each condition were obtained that were processed independently
2026-07-01 | E-MTAB-15224 | biostudies-arrayexpress
Project description:To investigate the effect of H2S on DNA methylation of plants submitted to drought stress, we performed transcriptome sequencing analysis in four experimental treatments: rice watered plants (control sample, C), rice plants watered with NaHS solution, as sulfide donor (sulfide treatment, S), rice plants subjected to drought stress (drought, D) and rice plants pretreated with NaHS and subjected to drought stress (S_D). Thus, 25-day-old plants grown in soil under physiological conditions were divided into two batches with one batch irrigated with water and the other with NaHS for 10 days, replacing the NaHS solution every two days. After this period, each batch was subsequently divided into two new batches and subjected to water irrigation or drought for another 15 additional days. At the end of the full treatment, four replicates of each condition were obtained that were processed independently
2026-07-01 | E-MTAB-15231 | biostudies-arrayexpress
Project description:Six weeks old Arabidopsis plants were transferred to a low CO2 (100 ppm) environment during 24 hours and compared to control plants kept under ambient CO2 conditions. Limited CO2 availability will cause higher rates of photorespiration and affect the plant redox homeostasis. We studied the transcriptomic impact of exposing plants to a lower CO2 environment to further eliculidate the signaling pathways during photorespiratory stress. After 6 weeks plants were transferred to low CO2 conditions (100 ppm) during 24 h or kept at 400 ppm CO2 (control condition). Design: 3 replicates x 2 conditions (low CO2 - ambient CO2)
2016-06-10 | E-GEOD-80158 | biostudies-arrayexpress
Project description:Hydrogen peroxide (H2O2) can act as a signaling molecule that influences various aspects of plant growth and development, including stress signaling and cell death. To unravel the molecular mechanisms that regulate the response towards an impact of increased H2O2 levels in plant cells, we focused on the photorespiration-dependent peroxisomal H2O2 production in Arabidopsis thaliana mutants lacking CATALASE2 (CAT2) activity (cat2-2) and screened for second-site mutations that attenuate the Fv'/Fm' decrease and lesion formation linked to the cat2-2 phenotype. A mutation in GLYCOLATE OXIDASE 1, encoding one of the two photorespiratory isoforms of glycolate oxidase rescued the cell death phenotype of cat2-2 plants under photorespiration-promoting conditions. Interestingly, introduction of gox2 into the cat2 background did not have the same effect and the double cat2 gox2 mutants were as affected as the parental cat2 mutants under conditions promoting photorespiration. Using a series of physiological, biochemical and metabolomic approaches we investigated the differential photorespiratory response of cat2 mutants underlied by the lack of GOX1 and GOX2. These differences are in line with the non-redundant functions of GOX1 and GOX2 which could be observed under enhanced photorespiration. Unstressed and stressed samples of cat2 gox1 and cat2 gox2 double mutants in triplicate
2016-06-10 | E-GEOD-77170 | biostudies-arrayexpress
Project description:Hydrogen peroxide (H2O2) is a potent signaling molecule influencing various aspects of plant growth and development. Its limited lifetime and specific production sites in the plant cell necessitate the existence of specialized mechanisms that relay H2O2-encoded information. To discover such mechanisms, we focused on peroxisomal H2O2 production triggered by enhanced photorespiration in Arabidopsis mutants lacking catalase activity (cat2-2), and looked for second-site mutations that attenuate the negative effects (Fv'/Fm' decline and lesion formation) of H2O2 build up. A mutation residing in the GRAS family transcriptional regulator SHORT-ROOT (SHR) was found to underlie the increased performance of cat2-2 knock-outs under photorespiratory stress. In contrast to shr, introduction of the scr mutation in cat2-2 background did not improve the photorespiratory performance of plants lacking peroxisomal catalase. The absence of SHR negatively affected the activity of the photorespiratory enzymes glycolate oxidase and catalase, which was accompanied with elevated glycolate content and inability to accumulate glycine under conditions promoting photorespiration. The transcriptome signature of cat2-2 shr-6 double mutants exposed to photorespiratory stress lacked jasmonate-dependent signaling components, otherwise induced in cat2-2. The photorespiratory phenotype of cat2-2 was found to be modulated by exogenous sugars both in the presence and absence of shr. Taken together, these findings highlight a crucial role for SHR in H2O2 signal transduction and stress tolerance. Three-week-old cat2-2 and shr-6/cat2-2 plants were exposed to photorespiratory conditions. Rosettes were sampled in three biological replicates before (T = 0) and after 24 hours (T = 24H) of the treatment. Total RNA was extracted with Spectrum Plant Total RNA Kit (Sigma) and submitted to Arabidopsis ATH1 microarray hybridization.
2016-06-10 | E-GEOD-66365 | biostudies-arrayexpress
Project description:Hydrogen peroxide (H2O2) can act as a signaling molecule that influences various aspects of plant growth and development, including stress signaling and cell death. Catalase deficient plants are pioneering systems which accumulate hydrogen peroxide (H2O2) from peroxisomal origin during photorespiratory challenges. Respiratory burst oxidase homologues D and F are known to participate in intracellular oxidative stress response launched in cat2 mutants (Chaouch et al., 2012). We studied the compared the transcriptional response of cat2 rbohD and cat2 rbohF double mutants versus the cat2 background to further adress their role during photorespiratory stress. After 3 weeks of growth, leaf tissue from the three different genotypes was harvested in triplicate.
2016-06-10 | E-GEOD-81516 | biostudies-arrayexpress
Project description:This ProteomeXchange dataset contains native mass spectrometry and intact-protein mass spectrometry data generated in support of the manuscript “A labile sulfur ligand in a three-cysteine-coordinated [2Fe-2S] cluster mediates sulfide sensing in NreB”. The dataset includes intact-protein MS measurements of purified His_NreB and His_NreB_PAS for molecular-mass verification, as well as native MS measurements of NreB_PAS under untreated, GSH-treated, GSH plus NaHS-treated, and pH-dependent conditions. These data were used to characterize Fe-S cluster-associated adduct states and to compare the distributions of species detected under different conditions. Raw data acquired on a Bruker Impact HD mass spectrometer and the corresponding deconvolution outputs generated using Bruker DataAnalysis 4.2 are included together with a deconvolution summary table.
2026-04-15 | PXD076861 | Pride
Project description:The high metabolic flux through photorespiration constitutes a significant part of the carbon cycle. Although, the major enzymatic steps of the photorespiratory pathway are well characterized, little information is available on the functional significance of photorespiration beyond carbon recycling. Particularly important in this respect is the peroxisomal catalase activity which removes photorespiratory H2O2 generated during the oxidation of glycolate to glyoxylate, thus maintaining the cellular redox homeostasis governing the perception, integration and execution of stress responses. By perfroming a chemical screen, we identified 34 small molecules that alleviate the negative effects of photorespiration in Arabidopsis thaliana mutants lacking photorespiratory catalase (cat2). The chlorophyll fluorescence parameter photosystem II maximum efficiency (Fv'/Fm') was used as a high-throughput readout. The most potent chemical that could rescue the photorespiratory phenotype of cat2 is a pro-auxin that contains a synthetic auxin-like substructure belonging to the phenoxy herbicide family which can be released in planta. The naturally occurring indole-3-acetic acid (IAA) and other chemically distinct synthetic auxins also inhibited the photorespiratory-dependent cell death in cat2 mutants, implying a role for auxin signaling in stress tolerance. We used global transcriptome profiling to characterize the effect of a novel pro-axin structure (2-(2,4-dichlorophenoxy)-N-[4-(isobutyrylamino)-3-methoxyphenyl]propanamide) on Arabidopsis Col0 seedlings 2-(2,4-dichlorophenoxy)-N-[4-(isobutyrylamino)-3-methoxyphenyl]propanamid (5 μM) was added to seven-day-old Arabidopsis Col0 seedlings grown in a 96-well plate setup. Control plants were treated with solvent (DMSO) only. RNA was extracted 24 h following chemical addition.
2013-11-02 | E-GEOD-52013 | biostudies-arrayexpress
Project description:Hydrogen peroxide (H2O2) can act as a signaling molecule that influences various aspects of plant growth and development, including stress signaling and cell death. To unravel the molecular mechanisms that regulate the response towards an impact of increased H2O2 levels in plant cells, we focused on the photorespiration-dependent peroxisomal H2O2 production in Arabidopsis thaliana mutants lacking CATALASE2 (CAT2) activity (cat2-2) and screened for second-site mutations that attenuate the Fv'/Fm' decrease and lesion formation linked to the cat2-2 phenotype. A mutation in the transcriptional regulator SHORT-ROOT (SHR) of the GRAS family rescued the cell death phenotype of cat2-2 plants under photorespiration-promoting conditions in a SCR-independent way, and provoked perturbation of photorespiratory metabolites. SHR deficiency boosted ascorbate levels and prevented the oxidation of the glutathione pool in cat2-2 background upon exposure to photorespiratory stress. These results reveal an unanticipated role for SHR as a regulator of cellular redox homeostasis. Unstressed and stressed samples of 4 genotypes (Col-0, cat2, shr and cat2 shr) in triplicate
2016-07-28 | E-GEOD-77017 | biostudies-arrayexpress