RNA-seq of mouse intestinal regenerative reprogramming

ABSTRACT: The intestinal epithelium constitutes a vital absorptive barrier that protects the host organism against luminal bacteria. Following tissue damage, intestinal epithelial cells are reprogrammed into a state characterized by YAP1 activation to facilitate barrier restoration. We demonstrate that mechanical fragmentation of intestinal organoids induces transient regenerative reprogramming, driving the formation of spheroid morphology, nuclear YAP1 accumulation, and the expression of regeneration-associated markers. We identify ALK4/ALK5 signaling as a regulator of the regenerative response and dissect the effects of the specific ALK4 and ALK5 ligands, Activin and TGF-β, thereby illustrating the importance of organoid models in dissecting regenerative responses and identifying novel signaling p

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Mus musculus

SUBMITTER: Laura Pikkupeura 

PROVIDER: E-MTAB-15918 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress