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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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K562 stimulated with TNFα with translation blocking using Puromycin. 12 samples were taken with ChronoSeq and barcodes with unique time-tags

ABSTRACT: We applied ChronoSeq to profile inflammatory responses in K562 cells stimulated with TNF-α, to capture the rapid NF-κB signaling cascade. 10 minute interval between samples including a 1 minute sampling duration. In addition Puromycin was added at a does of 5μg/ml for blocking translation. The purpose of this experiment was to measure TNF-α stimulation transcriptional program without translational feedback.

INSTRUMENT(S): Illumina NovaSeq X

ORGANISM(S): Homo sapiens

SUBMITTER: Kanishk Asthana

PROVIDER: E-MTAB-15947 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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