RNA-seq of human 293T cell line mitochondrial small RNA transcriptome
Ontology highlight
ABSTRACT: This study aimed to determine whether human nuclear-encoded tRNAs can enter mitochondria and to assess their potential involvement in mitochondrial protein translation. We isolated cytoplasmic fractions, intact mitochondria, and mitoplasts from human HEK293T cells and performed small RNA sequencing to profile tRNAs and other small RNAs. The mitoplast samples enabled the distinction between RNAs associated with the mitochondrial surface and those localized within the mitochondrial interior. In parallel, mitochondrial ribosomes were purified from the mitochondrial fraction to obtain mitochondrial ribosome-associated tRNAs. This dataset enables the characterization of mitochondrial small RNAs, the assessment of nuclear-encoded tRNA localization within mitochondria, and the evaluation of their associations with the mitochondrial translation machinery.
INSTRUMENT(S): Illumina NovaSeq X
ORGANISM(S): Homo sapiens
SUBMITTER: Cao Lab
PROVIDER: E-MTAB-15948 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
ACCESS DATA