HM-DyadCap – Capture and Mapping of Asymmetric 5-Hydroxymethylcytosine/5-Methylcytosine CpG Dyads in Mammalian DNA

ABSTRACT: 5-Methylcytosine (mC) and 5-hydroxymethylcytosine (hmC) are the two main epigenetic modifications of mammalian DNA, and play crucial roles in cell differentiation, development, and tumorigenesis. Both modifications co-exist with unmodified cytosine in palindromic CpG dyads in different strand-symmetric and -asymmetric combinations, each having unique regulatory potential. To facilitate investigating the individual functions of such dyad modifications, we here report HM-DyadCap. This method employs MECP2 HM – an evolved methyl-CpG-binding domain of the mC reader MECP2 – for the simple capture and sequencing of DNA fragments containing the CpG dyad hmC/mC that frequently occurs in mouse embryonic stem cell and brain genomes. In vitro binding studies reveal a high discrimination of MECP2 HM a

INSTRUMENT(S): Illumina NovaSeq X

ORGANISM(S): Mus musculus

SUBMITTER: Marlon Zambrano 

PROVIDER: E-MTAB-15961 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress