Bulk K562 and EL4 cells mixed with 12 Time-Tags for verification (QC) for ChronoSeq Beads
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ABSTRACT: We checked our ability to uniquely identify each Time-Tag. We did this by mixing beads from odd numbered Time-Tags with human cells and even numbered Time-Tags with mouse cells manually in bulk. These beads were then washed and combined for Reverse Transcription, library preparation and sequencing. Time-Tag resolved cleanly into either human or mouse dominant with 1.6% or less mixed beads detected for each Time-Tag.
INSTRUMENT(S): Illumina NovaSeq X
ORGANISM(S): mixed sample
SUBMITTER: Kanishk Asthana
PROVIDER: E-MTAB-15965 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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