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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Processed RNA-seq of 95 lung adenocarcinoma samples

ABSTRACT: The AllPrep DNA/RNA mini kit (Qiagen) was used for RNA extraction following manufacturer’s instructions. Libraries were prepared using the Illumina TruSeq stranded mRNA protocol and sequenced on the NovaSeq 6000 system at the Center for Translational Genomics (www.ctg.lu.se) in Lund, Sweden. Demultiplexing was performed using the bcl2fastq2 software v2.18.0.12 (Illumina) with default settings and the quality was checked with FastQC v0.11.3. Reads were mapped to the GRCh38 reference genome using the HISAT2 software v2.1.0 and annotation files from release 103. Finally, RNA-sequencing (RNA-seq) expression data in FPKM were calculated with StringTie v1.3.4d.

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Homo sapiens

SUBMITTER: Deborah F. Nacer

PROVIDER: E-MTAB-16082 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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