Transcriptional Subtypes on Immune Microenvironment and Predicting Postoperative Recurrence and Metastasis in Pheochromocytoma and Paraganglioma

ABSTRACT: Tumor tissues from 87 patients with pheochromocytoma and paraganglioma (PCPG) were collected from surgical resection specimens that had been archived as formalin-fixed paraffin-embedded (FFPE) blocks. All patients had complete clinicopathological information and follow-up records, including postoperative recurrence and distant metastasis status. For each case, one representative tumor block was selected by a pathologist, and total RNA was extracted from FFPE sections using a standard deparaffinization and RNA purification protocol. RNA libraries were prepared for bulk RNA sequencing using an FFPE-compatible workflow and sequenced on a BGISEQ platform to generate paired-end reads. This study aimed to define transcriptional subtypes of PCPG, to compare immune-microenvironment–related gene expression among subtypes, and to link expression patterns with clinical outcomes (recurrence/metastasis).

INSTRUMENT(S): BGISEQ-500

ORGANISM(S): Homo sapiens

SUBMITTER: Yang Liu 

PROVIDER: E-MTAB-16109 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress