Transcriptome sequencing on a time series of potato infected with Globodera pallida to explore the sex determination
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ABSTRACT: We explored the sex determination of Globodera pallida. To do so, we grew cuttings of the potato cultivar Desiree on Gamborg B5 medium supplemented with either 1.5 g/L or 20 g/L sucrose. 14-day-old cuttings were inoculated with 100 G. pallida E400 Rookmaker juveniles. Infected root tissue was harvested at 1, 3, 6, and 9 dpi. Also, a subsample of the ppJ2 was kept for each batch. The experiment contains four time-separated batches. Transcriptome sequencing was performed by BGI Hong Kong. Two samples (B_4.2_1,5; B_4.3_1,5) were not taken along for sequencing because of a failed library prep.
INSTRUMENT(S): DNBSEQ-G400, Maxwell 16 LEV-plant RNA kit
ORGANISM(S): Globodera pallida
SUBMITTER: Mark Sterken
PROVIDER: E-MTAB-16117 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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