Unknown,Transcriptomics,Genomics,Proteomics

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Single-cell RNA sequencing data of Ly49⁺ CD8 T cells treated with anti-CD80/86 antibody and control Ig

ABSTRACT: This study aims to investigate the functional role of Ly49+CD8+T cells in allogeneic immune responses during cardiac transplantation. Using a murine heart transplant model and mixed lymphocyte reactions, we analyzed how these cells respond under CD80,86-CD28 co-stimulation blockade. The results revealed that Ly49+CD8+T cells exhibit distinct proliferative and cytotoxic features and contribute to immune regulation through Qa-1 interaction and inhibitory receptors such as LAG-3 and CTLA-4. This research provides new insights into the immunoregulatory mechanisms that promote graft tolerance and may inform future strategies to prevent transplant rejection.

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Mus musculus

SUBMITTER: Gong Yu

PROVIDER: E-MTAB-16148 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Project description:Inducible co-stimulator (ICOS) interaction with its ligand (ICOSL) is involved in several T cell effector functions. While blockade of ICOS:ICOSL interaction in chronic graft versus host disease (GVHD) seems benefi cial, results for acute GVHD remain controversial. To further elucidate its role in acute GVHD, C57BL / 6 mice were lethally irradiated and reconstituted with allogeneic spleen cells in the absence or presence of ICOSL-blocking mAb. Mice reconstituted with allogeneic spleen cells experienced severe GVHD and died untreated within 6 M-bM-^@M-^S 9 days after transplantation. Mice treated with an anti-ICOSL mAb starting from day 3 after transplantation gained weight again and survived for at least additional 12 days, although the treatment was already stopped at day 11 after transplantation. In contrast, the anti-ICOSL treatment starting from day 0 did not prevent GVHD. The diff erence between therapeutic (day 3) and prophylactic (day 0) anti-ICOSL treatment was independent of CD25 + CD4 + regulatory T cells since their depletion did not abrogate the therapeutic eff ect of ICOSL blockade. Microarray analysis revealed IFN- M-NM-3 and chemokine up-regulation in spleen cells of prophylactically treated mice, emphasizing kinetic dependence of acute GVHD modulation via blockade of ICOS:ICOSL interaction. B6 recipients were irradiated with 10.0 Gray, administered from a 137 Cs source. Splenocytes from C3H mice were prepared as single cell suspensions in PBS, depleted of red blood cells and counted. 2 M-bM-^@M-^S 3 M-CM-^W 10^7 C3H splenocytes in a volume of 200 M-NM-<l were transplanted into B6 recipients via tail vein injection (4 mice per group per experiment) 4 M-bM-^@M-^S 6 h after irradiation. Mice in the treatment group with anti-ICOSL mAb and their respective controls, received 500 M-NM-<g mAb i. p starting at day 0 or day 3, followed by subsequent injections of 200 M-NM-<g of mAb every other day. At day 4 after transplantation RNA of spleen cells was prepared and subjected to microarray analysis. Combined RNA from allogeneic transplanted mice was hybridized onto 2 independent arrays.

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Single-cell RNA sequencing data of Ly49⁺ CD8 T cells treated with anti-CD80/86 antibody and control Ig

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