Unknown,Transcriptomics,Genomics,Proteomics

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RNA-seq of Vibrio cholerae wild-type and dbfS, dbfQ, and dbfR(D51V) mutant strains

ABSTRACT: RNA-seq was performed to characterize transcriptional changes governed by the DbfRS two-component signaling system in Vibrio cholerae. The experiment compared global gene expression in wild-type and mutant strains lacking dbfS or dbfQ, as well as a phospho-dead dbfR(D51V) variant. Cultures were grown in M9 minimal medium to mid-log phase before RNA extraction. Libraries were sequenced and mapped using HISAT2, and gene quantification was performed with featureCounts followed by normalization and differential expression analysis using edgeR. The resulting data define the DbfR regulon and the transcriptional consequences of DbfRS pathway activation.

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Vibrio cholerae

SUBMITTER: Andrew Bridges 

PROVIDER: E-MTAB-16165 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

A small periplasmic protein governs broad physiological adaptations in <i>Vibrio cholerae</i> via regulation of the DbfRS two-component system.

Nguyen Emmy E   Agbavor Charles C   Steenhaut Anjali A   Pratyush M R MR   Hiller N Luisa NL   Cahoon Laty A LA   Mikheyeva Irina V IV   Ng Wai-Leung WL   Bridges Andrew A AA  

bioRxiv : the preprint server for biology 20250324

Two-component signaling pathways allow bacteria to sense and respond to environmental changes, yet the sensory mechanisms of many remain poorly understood. In the pathogen <i>Vibrio cholerae</i>, the DbfRS two-component system controls the biofilm lifecycle, a critical process for environmental persistence and host colonization. Here, we identified DbfQ, a small periplasmic protein encoded adjacent to <i>dbfRS</i>, as a direct modulator of pathway activity. DbfQ directly binds the sensory domain  ...[more]

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