Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Serum starvation affects the transcriptomic and proliferative response to ACTH in primary cultures of rat adrenocortical cells

ABSTRACT: This study investigated how serum composition modulates ACTH-induced gene expression in primary rat adrenocortical cells. Cells were cultured in standard or charcoal-stripped serum (which removes steroids along with other serum components such as growth factors and lipids), then stimulated with ACTH (10 nM) for 24 hours. Microarray analysis revealed that ACTH under serum-depleted conditions activated 532 differentially expressed genes (456 upregulated, 76 downregulated), compared to only 220 genes under standard conditions. Key steroidogenic genes including Cyp11a1, Cyp11b1, Star, and Scarb1 showed markedly enhanced upregulation in the serum-depleted condition. Functional enrichment analysis demonstrated that ACTH preferentially activated steroid biosynthesis and lipid metabolic pathways while suppressing cell cycle and extracellular matrix-related genes, particularly under serum depletion. These findings demonstrate that serum composition critically shapes ACTH responsiveness, revealing multifactorial regulation of adrenocortical cell function beyond steroid feedback alone.

ORGANISM(S): Rattus norvegicus

SUBMITTER: Marcin Rucinski

PROVIDER: E-MTAB-16213 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Serum starvation affects the transcriptomic and proliferative response to ACTH in primary cultures of rat adrenocortical cells

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