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RNA-seq of Arabidopsis thaliana Col-0 wildtype and single mutants crwn1, crwn2, crwn3, crwn4 and kaku4 in short-day conditions collected at 150 days after sowing

ABSTRACT: RNA-seq experiment to characterize transcriptome changes associated with premature aging in Arabidopsis nuclear-envelope mutants. Col-0 wild type and single mutants in CRWN1, CRWN2, CRWN3, CRWN4 and KAKU4 were grown on soil under short-day conditions (8 h light / 16 h dark) until 150 days after sowing, a stage at which nuclear-envelope mutants display strong rosette senescence and loss of meristem activity. Entire rosettes were harvested, immediately frozen, and pooled per genotype to capture whole-plant vegetative transcriptional states. Total RNA was extracted from rosette tissue, mRNA was enriched, and strand-specific Illumina libraries were prepared and sequenced as paired-end short reads. The resulting dataset is intended to (i) compare global gene-expression profiles between wild type and individual nuclear-envelope mutants at late vegetative stages, (ii) identify pathways associated with progeroid-like phenotypes, and (iii) provide a resource for linking nuclear architecture defects to age-related transcriptional drift in plants.

INSTRUMENT(S): N/A, Thermocycler; magnetic rack for SPRI bead clean-up, Illumina NovaSeq 6000, Liquid-nitrogen grinding tools; benchtop centrifuge, Controlled-environment growth chamber (short-day; 8 h light / 16 h dark)

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Oscar Juez

PROVIDER: E-MTAB-16251 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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