Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Genome-wide KAKU4-eYFP ChIP-seq of Arabidopsis thaliana kaku4 complemented and untagged control plants at seedling (10 days) and 120-day rosette stages

ABSTRACT: Chromatin immunoprecipitation sequencing (ChIP-seq) was used to map the genome-wide binding profile of the inner nuclear envelope protein KAKU4 in Arabidopsis thaliana. A functional ProKAKU4:KAKU4-eYFP transgene (Goto et al., Plant Cell 2014), which complements the kaku4-3 mutant, and an untagged kaku4 (SALK_076754) control line were analysed. Young samples consisted of 10-day-old seedlings (n = 3 biological replicates per genotype, pooling ~12 seedlings per replicate). Old samples consisted of 120-day short-day rosettes, where senescence leaves from 3–4 plants were pooled per replicate (n = 3 replicates). Together enabling comparison of KAKU4 occupancy between juvenile and long-term aged tissues.

INSTRUMENT(S): Thermocycler; SPRI magnetic bead rack; Qubit fluorometer; Agilent Bioanalyzer, Controlled-environment growth chambers (long-day and short-day), Illumina NovaSeq 6000, Dissection tools; liquid nitrogen; −80 °C freezer, Branson Digital Sonifier; refrigerated benchtop centrifuge; rotator; magnetic rack

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Oscar Juez

PROVIDER: E-MTAB-16266 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Dataset Information

Genome-wide KAKU4-eYFP ChIP-seq of Arabidopsis thaliana kaku4 complemented and untagged control plants at seedling (10 days) and 120-day rosette stages

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