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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcriptomic characterization of circadian clock gene-mutated human pluripotent stem cell lines

ABSTRACT: To explore the non-canonical functions of core clock proteins in ESCs, we generated CRISPR/Cas9-edited isogenic human ESC lines carrying targeted truncations of CLOCK, BMAL1, NR1D1, and/or NR1D2. Comprehensive characterization and transcriptomic analyses demonstrated changes in gene expression associated with pluripotency and differentiation, despite the absence of rhythmic activity.

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Homo sapiens

SUBMITTER: Hyun Jung Kim

PROVIDER: E-MTAB-16296 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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