RNA-seq of human beta-cell line EndoC-betaH1 with and without single nucleotide replacement in the human TIA1 gene (E384K mutation)

ABSTRACT: Single nucleotide replacement in the human TIA1 gene (E384K) in human EndoC-βH1 cells was achieved by PE according to a previous publication (Anzalone, Randolph, Davis et al., 2019). CRISPR PE guide RNA (pegRNA) and nicking sgRNA (ngRNA) sequences were designed using the web resources available at https://drugthatgene.pinellolab.partners.org/primevar. PE plasmid is pCMV-PE2-P2A-GFP (Addgene plasmid #132776) expressing Cas9 H840A with co-translational GFP expression. PegRNA plasmid was constructed by ligation of annealed oligonucleotides including sequences of spacer, scaffold, and 3' extension into BsaI-digested pU6-pegRNA-GG-acceptor (Addgene plasmid # 132777). Plasmids expressing ngRNAs were constructed by ligation of annealed oligonucleotides into U6-BsmBIcassette-Sp-sgRNA BPK1520 (Addg

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Homo sapiens

SUBMITTER: Xuan Wang 

PROVIDER: E-MTAB-16316 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress