Project description:This microarray study aimed at evaluating the impact of mosquito chemical environment on the selection of insecticide resistance mechanisms. Here the mosquito Aedes aegypti was used as a model to perform a laboratory experiment combining mosquito larvae exposure to a sub-lethal dose of xenobiotic and their selection with the insecticide permethrin. After ten generations, bioassays and a transcriptome profiling with the 15K microarray Aedes detox chip plus microarray were performed comparatively on all strains.
Project description:This microarray study aimed at comparing constitutive gene expression levels between an Aedes aegypti insecticide-resistant strain (Imida-R) selected at the larval stage with the neonicotinoid insecticide imidacloprid for 10 generations and the parental strain (Bora-Bora) susceptible to all insecticides. Strains comparison was performed at both larval (4th stage larvae) and adult (3 days-old adults females, non-blood fed) stages.
Project description:Bacillus thuringiensis israelensis (Bti) toxins are increasingly used for mosquito control, but little is known about the precise mode of action of each of these toxins, and how they interact to kill mosquito larvae. By using RNA sequencing, we investigated change in gene transcription level and polymorphismvariations associatedwith resistance to each Bti Cry toxin and to the full Bti toxin mixture in the dengue vector Aedes aegypti. The upregulation of genes related to chitin metabolismin all selected strain suggests a generalist, non-toxin-specific response to Bti selection in Aedes aegypti. Changes in the transcription level and/or protein sequences of several putative Cry toxin receptors (APNs, ALPs, α-amylases, glucoside hydrolases, ABC transporters) were specific to each Cry toxin. Selective sweeps associated with Cry4Aa resistancewere detected in 2 ALP and 1 APNgenes. The lack of selection of toxin-specific receptors in the Bti-selected strain supports the hypothesis that Cyt toxin acts as a receptor for Cry toxins in mosquitoes.
Project description:This microarray experiment aimed at studying the response of Aedes aegypti 4th stage-larvae to various xenobiotics, including insecticides, polycyclic aromatic hydrocarbons, herbicides and heavy metals.
Project description:This study aimed at comparing gene transcription using microarrays and protein expression using 2D-DIGE between an Aedes aegypti insecticide-resistant strain (LiTOX) selected for 28 generations at the larval stage with field-collected leaf litter containing persistent Bacillus thuringiensis var. israelensis (Bti) toxins and the parental strain (Bora-Bora) susceptible to all insecticides. We focused on the tissue where the mode of action of the insecticide takes place: the midgut of the larvae.
Project description:Transcriptome profiling of two pyrethroid resistant strains of Aedes aegypti compared to a susceptible strain. The two resistant strains are from Cuba and Cayman Island and the susceptible strain from New Orleans.
Project description:Transcriptome profiling of pyrethroid resistant field populations of Anopheles funestus from Malawi and Mozambique compared to a susceptible lab strain FANG
Project description:The transcriptional profile of pyrethroid resistant Anopheles arabiensis from Zanzibar. Anopheles arabiensis from Pemba Island, exposed (Survivors) and non-exposed (Pemba) to a discriminating dose of the pyrethroid lambda-cyhalothrin were compared to two insecticide susceptible strains from Zanzibar island (Unguja) and Dar es Salaam (Dar).
Project description:The transcriptional profile of Anopheles arabiensis collected from a pyrethroid resistant area on Pemba Island collected in 2011 was compared to that of a lab susceptible colony of Anopheles arabiensis (MOZ).