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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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CUT&ID of 8 targets in HCT116

ABSTRACT: We developed CUT&ID, that simultaneously profiles genome-wide DNA and protein associations of any endogenous target in its native state, from one sample in a single streamlined workflow, and with no need of cloning or transgenesis. CUT&ID is enabled by a single fusion protein combining TurboID, protein A/G, and micrococcal nuclease, which drives sequential target recognition, proximal proteinity biotinylation and targeted DNA cleavage in living cells. We applied CUT&ID to CTCF, SMARCA4, H3K27ac, TCF7L2, b-catenin, JUN, RAD21, and HDAC1 in HCT116 cells.

INSTRUMENT(S): NextSeq 2000

ORGANISM(S): Homo sapiens

SUBMITTER: Anna Nordin

PROVIDER: E-MTAB-16382 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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