Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcriptomic effect of parental exposure to a crude oil water-soluble fraction in Atlantic cod (Gadus morhua) early life stages

ABSTRACT: This study investigates how parental exposure to the water-soluble fraction (WSF) of crude oil influences transcriptomic profiles in Atlantic cod (Gadus morhua) offspring. Fish were exposed to WSF for 20 days prior to spawning, and four offspring groups were generated by fertilization: control (unexposed parents), maternally exposed, paternally exposed, and biparentally exposed. Transcriptome analyses of embryos were performed at key developmental stages at 0, 2, 4 and 21 days post feralization (dpf) and the observed changes were correlated with phenotypic traits such as cardiac function and morphological measurements. Offspring groups from exposed females died before hatching. Transcriptome analyses indicated severe suppression or absence of expression of genes involved in early development in the maternally exposed groups. In contrast, paternal exposure had minimal impact on gene expression, and no phenotypic abnormalities were observed at hatching. Nonetheless, reduced survival in early larval stages suggested sublethal toxicity of crude oil.

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Gadus morhua

SUBMITTER: Fekadu Yadetie

PROVIDER: E-MTAB-16384 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Publications

Maternal and paternal crude oil exposure differentially shapes early developmental transcriptomes and survival outcomes in Atlantic cod (Gadus morhua).

Erhart Claudia C Nahrgang Jasmine J Odei Derrick Kwame DK Frantzen Marianne M Sørensen Lisbet L Creese Mari Egeness ME Puvanendran Velmurugu V Hansen Øyvind Johannes ØJ Hansen Bjørn Henrik BH Meador James P JP Yadetie Fekadu F

Marine pollution bulletin 20260210

Understanding the long-term biological consequences of crude oil exposure on marine fish is essential for the sustainability of ecologically and economically important species such as Atlantic cod (Gadus morhua). While the direct effects of crude oil on early life stages are well documented, adult reproductive vulnerability and intergenerational consequences remain poorly understood, despite their pivotal role in spawning stock viability. Paternal contributions to next-generation outcomes are pa ...[more]

PMID: 41671699

Similar Datasets

Project description:This study presents statistical analyses of gene expression in 5, 10 and 15 day post-fertilization (dpf) embryos of the teleost Fundulus heteroclitus treated with control vehicle (DMSO) or a potent non-ortho-PCB (PCB-126; 3,3’,4,4’,5-pentachlorobiphenyl). The embryos were from two populations: a clean, reference population (SC, Scorton Creek, MA USA) and a polluted Superfund population (N, New Bedford Harbor, MA USA). For each site, eggs from 8 females (~1100 total) were fertilized using minced testes from 5 males. After non-fertile eggs were culled, embryos were exposed to vehicle (DMSO; 0.1%) or PCB-126 (50 nM) in filtered seawater (salinity 25 part per thousand, 65 embros per 20 ml in glass petri dish) for 4 hr at 20º C. After exposure, the embryos were washed in filtered seawater and incubated at 20º C under a 14-h light, 10-h dark cycle. At 5-, 10-, and 15-dpf, embryos were collected as three pools of 20 embryos from each treatment group and flash frozen in liquid nitrogen and stored at -80º C until used for RNA isolation. A loop design was used for the microarray hybridizations where each sample is hybridized to 2 arrays using both Cy3 and Cy5 labeled fluorophores. We used three loops in which each loop consisted of Cy3- and Cy5-labeled embryo aRNAs from 12 samples: one sample from each population-treatment-time combination. Within a population-treatment-time, embryos were randomly assigned to one of the three loops. In total, 36 embryos were hybridized to 36 microarrays. The loops formed were SC5C→ SC5P → NBH5C→ NBH5P →SC10C→ SC10P→ NBH10C→ NBH10P → SC15C→ SC15P → NBH15C → NBH15P → SC5C, where each arrow represents a separate hybridization (array) with the biological sample at the base of the arrow labeled with Cy3 and the biological sample at the head of the arrow labeled with Cy5. SC represents the Scorton Creek, Sandwich, MA population (reference), NBH represents the New Bedford Harbor, MA population, C represents control dose (DMSO), P represents the PCB-126 dose, 5 represents 5 dpf, 10 represents 10 dpf and 15 represents 15 dpf.

Dataset Information

Transcriptomic effect of parental exposure to a crude oil water-soluble fraction in Atlantic cod (Gadus morhua) early life stages

Publications

Maternal and paternal crude oil exposure differentially shapes early developmental transcriptomes and survival outcomes in Atlantic cod (Gadus morhua).

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets