PacBio Iso-seq of Lupinus angustifolius L. leaf and stem transcriptomes

ABSTRACT: The aim of this experiment was to obtain full length transcript sequences of genes expressed in leaves and stems of narrow-leafed lupin (Lupinus angustifolius L.) using PacBio Iso-Seq. Total RNA was isolated from leaves and stems of five narrow-leafed lupin (Lupinus angustifolius L.) genotypes with contrasting alkaloid regulation, including an iucundus low-alkaloid line (83A:476), a bitter Iucundus line (P27255) and three Bryansk low-alkaloid lines carrying the Iucundus-type RAP2-7 allele: 95826 (Bryanskij-35), 95927 (Bryanskij-123) and 95928 (Bryanskij-237/83). Plants, in five replicates per genotype, were grown under controlled conditions optimal for growth, and leaf and stem samples were collected at flowering stage. For each genotype and organ, total RNA from five biological replicates

INSTRUMENT(S): Sequel II

ORGANISM(S): Lupinus angustifolius

SUBMITTER: Magdalena Kroc 

PROVIDER: E-MTAB-16429 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress