RNA sequencing–based determination of the RNA interactome of wild-type AKAP1 and its deletion mutants deficient for the KH or Tudor domain.
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ABSTRACT: AKAP1 is highly expressed in glioblastoma and is required for tumor growth. We therefore analyzed the AKAP1 RNA interactome in glioblastoma cells. Native RNA immunoprecipitation followed by high-throughput sequencing (RIP-Seq) was performed in U87MG cells transiently expressing V5-tagged mouse wild-type AKAP1, as well as deletion mutants lacking either the KH (Δ563–630) or Tudor (Δ709–786) domain, to assess domain-specific RNA binding.
INSTRUMENT(S): Illumina NovaSeq 6000
ORGANISM(S): Homo sapiens
SUBMITTER: Domenico Memoli
PROVIDER: E-MTAB-16473 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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