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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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MRNA-Seq of Danio rerio exposed to different concentrations of the fungicide boscalid against untreated control groups

ABSTRACT: In the present study transcriptome analysis was employed to investigate the early molecular responses to exposure to the fungicide boscalid (CAS 188425-85-6). Zebrafish embryos were exposed to boscalid according to OECD guidelines (OECD test No. 236). At the end of exposure time (96 hours), simultaneous RNA and protein extraction from 10 embryos was performed using a Macherey & Nagel RNA/protein extraction kit. The obtained RNA extracts were sequenced using Illumina NovaSeq 6000 System and the obtained sequences went through bioinformatic analysis pipeline to Identify and count the detected gene sequences followed by differential gene expression analysis. Finally, potential substance specific biomarker candidates were refined and selected based on the differential expression patterns and the biological functions investigation of the detected DEGs.

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Danio rerio

SUBMITTER: Jule Bäcker

PROVIDER: E-MTAB-16491 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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