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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Human Embryonic Stem Cells Polysomes

ABSTRACT: H1s are induced to differentiate into the trophoblast (TE) lineage, and polysome analysis performed at 0hr (Undifferentiated), 12hr TE and 24hr TE. The whole cell extract (WCE) is a cytoplasmic lysate of the cell . G1-G4 represent RNAs that were found in different fractions of a sucrose gradient, which represents monosomes, polysomes, etc. Thus the WCE is used as the denominator so that for a particular transcript, we can express G1, for example, as a fraction of the total RNA.

ORGANISM(S): Homo sapiens

SUBMITTER: Shen Mynn Tan

PROVIDER: E-MTAB-1653 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Divergent LIN28-mRNA associations result in translational suppression upon the initiation of differentiation.

Tan Shen Mynn SM Altschuler Gabriel G Zhao Tian Yun TY Ang Haw Siang HS Yang Henry H Lim Bing B Vardy Leah L Hide Winston W Thomson Andrew M AM Lareu Ricky R RR

Nucleic acids research 20140523 12

LIN28 function is fundamental to the activity and behavior of human embryonic stem cells (hESCs) and induced pluripotent stem cells. Its main roles in these cell types are the regulation of translational efficiency and let-7 miRNA maturation. However, LIN28-associated mRNA cargo shifting and resultant regulation of translational efficiency upon the initiation of differentiation remain unknown. An RNA-immunoprecipitation and microarray analysis protocol, eRIP, that has high specificity and sensit ...[more]

PMID: 24860167

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2014-07-21 | E-MTAB-1652 | biostudies-arrayexpress

Mouse cardiac tissue, polysomes and monosomes: Vehicle treated control vs. 25mg/kg DMNQ

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2010-08-06 | E-GEOD-17830 | biostudies-arrayexpress

Mouse cardiac tissue, polysomes and monosomes: Vehicle treated control vs. 15mg/kg doxorubicin

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Study investigating efficacy of a medicine lanreotide on decrease of chemotherapy induced diarrhoea (CID) in patiens with cancer of colon and rectum.

Project description:Primary objectives: Definition of efficacy of lanreotid in the therapy of CID which is defined as a rate of regression of CID to grade G1 from the entry value (grade G3 or G4) according to CTCAE v.4.03 Primary endpoints: Paremeter of efficacy is the rate of regression of CID to grade G1 from the entry value (grade G3 or G4) during 7 days from the first administration of the investigational medicinal product.

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Characterization of G-quadruplexes in the rice genome

Project description:DNA G-quadruplex (G4) is a non-canonical four-stranded nucleic acid structure functioning in various biological processes in mammals. G4 ChIP-seq assay, In this study, we developed a robust BG4-DNA ChIP-seq for global identification of DNA G4 in rice..We found that G4s harbored some functional motifs for binding of certain trans-factors, were co-localized with R-loops and DHSs in the rice genome. Importantly, G4s exhibited differential effects on DNA methylation between TE and non-TE genes in rice. Especially, G4 intensity-dependent enrichment occurred between DNA 5mC and 6mA.These DNA-related features suggest potential regulatory roles of G4 in rice. Thus, our study will pave the way for prompting intensive characterization towards identifying novel functions of G4 in plants, especially for agronomic important crops.

2021-07-01 | GSE132775 | GEO

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Project description:We analyzed the transcriptional signatures of mouse bone marrow-derived macrophages (BMDM) at different times after infection with promastigotes of the protozoan parasite Leishmania major. GeneChip Mouse Gene 1.0 ST arrays were used to analyse global changes in gene transcripts to generate a pool of genes that was statistically significant (p-value < 0.05) and a fold change cut-off of 1.5 in at least one of the five infected samples versus non-infected samples. Three independent biological replicates for each biological condition (Non infected "NI", infected with lived parasite "P" or "heat inactivated parasite "Kp") at 1h (T1), 3(T3), 6(T6), 12 (T12) and 24hours (T24) were performed. The Non infected (NI) at 0 hour (T0) biological condition were used as an additional control.

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Gene Expression data from mouse bone marrow derived macrophages infected by the promastigote form of Leishmania major parasite (P) or Killed parasite (Kp) during a time course of infection

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2012-12-19 | E-GEOD-31997 | biostudies-arrayexpress

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