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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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RNA-seq of hiPSC-ECs from HNF1A-MODY patient and corrected isogenic control lines

ABSTRACT: The experiment aims to find differences in gene expression in endothelial cells due to a heterozygous mutation in HNF1A, which cause maturity-onset diabetes of the young (MODY). For that purpose human induced pluripotent stem cell (hiPSCs) line from an HNF1A-MODY patient was repaired using CRISPR/Cas9, thus generating two isogenic (patient-specific) control lines. The hiPSCs from patient and the respective control lines were differentiated toward endothelial cells, and the gene expression in these cells was compared.

INSTRUMENT(S): C1000 Touch PCR thermal cycler (Bio-Rad), NextSeq 2000

ORGANISM(S): Homo sapiens

SUBMITTER: Neli Kachamakova-Trojanowska

PROVIDER: E-MTAB-16548 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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