Amplicon sequencing of splicing reporter mini-genes in a massively parallel reporter assay

ABSTRACT: A library of cassette exon splicing reporter mini-genes was cloned under the control of five different promoters and tested, alongside pharmacological treatments affecting transcription, by transfection into HEK293 cells, isolation of the mRNA and targeted high-throughput sequencing of the reporter constructs to determine the effect of promoter identity and pharmacological treatment on splicing behavior.

INSTRUMENT(S): Illumina NovaSeq X

ORGANISM(S): Homo sapiens

SUBMITTER: Martin Mikl 

PROVIDER: E-MTAB-16553 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress