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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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MRNA-Seq of R. subcapitata exposed to different concentrations of the zinc oxide nanoparticle NM-110 against untreated control groups

ABSTRACT: n the present study transcriptome analysis was employed to investigate the early molecular responses to exposure to the zinc oxide nanoparticle NM-110. R. subcapitata was exposed to the ZnO NP NM-110 according to OECD guidelines (OECD test No. 201). At the end of exposure time (72 hours), simultaneous RNA and protein extraction was performed using a newly established RNA extraction protocol combinded with the Macherey & Nagel RNA/protein extraction kit. The obtained RNA extracts were sequenced using Novaseq6000 system with a read length of 150 base pairs in paired-end run mode and the obtained sequences went through bioinformatic analysis pipeline to identify and count the detected gene sequences followed by differential gene expression analysis and overrepresentation analysis

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Raphidocelis subcapitata

SUBMITTER: Melina Schöpker

PROVIDER: E-MTAB-16813 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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