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Micro-4C for investigating the interaction among HS2 core element and all other regions in β-globin locus in K562 and HL-60 cells

ABSTRACT: GATA1-binding sites constituted essential functional elements within the HS2 enhancer, enabling transcription factor recruitment, enhancer activation, and long-range chromatin interactions at the β-globin locus. In K562 cells, a human erythroleukemia line that expresses fetal and embryonic globin genes and possesses an active β-globin LCR, GATA-1 occupancy at HS2 was demonstrated by previous study. We performed micro-4C to investigate the interactions among HS2 core element and all other regions in β-globin locus in K562 and HL-60 myeloid leukemia cells (as a negative control). Erythroid-specific DNA interactions can be readily detected

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Homo sapiens

SUBMITTER: Yihang Shen

PROVIDER: E-MTAB-16830 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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