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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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ATAC-seq time-course profiling upon Wnt stimulation in ΔCTNNB1 HEK293T cells

ABSTRACT: Wnt/β-catenin signaling is generally thought to activate transcription primarily through pre-accessible regulatory elements. However, we previously identified cases in which β-catenin associates with initially inaccessible chromatin regions that subsequently gain accessibility (E-MTAB-12076, Pagella et. al., (2023) Cell Systems). To determine whether stimulus-dependent chromatin opening requires β-catenin, we performed ATAC-seq in β-catenin knockout (ΔCTNNB1) human embryonic kidney 293T (HEK293T) cells, both prior to and 4 hours following pathway activation.

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Homo sapiens

SUBMITTER: Tamina Weiss

PROVIDER: E-MTAB-16975 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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ATAC-seq experiment at four time points in HEK293 and hESC cells, assessing the time-resolved response on chromatin accessibility upon Wnt/β-catenin signaling activation

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