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Light-based footprinting of a eukaryotic genome

ABSTRACT: Whole genome UV footprinting in yeast using deamination sequencing (Deam-seq), wherein photoproducts induced by high-dose UVC irradiation are revealed as mutations. In Deam-seq, cytosines within cyclobutane pyrimidine dimers (CPDs) are converted to uracil by rapid deamination at elevated temperature. Following CPD repair by photolyase, samples were sequenced at ultra-deep (>7,000×) coverage, enabling calculation of per-base damage fractions (mutant allele frequencies). By comparing cellular and naked DNA, this approach can pinpoint genomic sites bound by proteins that modulate UV damage. The samples were irradiated with UVC on ice, for 45 seconds. Raw fastq files are available in ENA (accession: PRJEB111787).

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Linnéa Ögren

PROVIDER: E-MTAB-17008 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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